Cloning into transfection plasmids, with or without reporter genes is offered starting from cDNAs and plasmids provided by the customer or by accessing cDNAs and plasmids provided by COMT. This service includes the production of mutated constructs by site-directed mutagenesis of amino acid residues, constructs carrying specific deletions or constructs containing fragments of the cDNA of the gene of interest.

References

Dr Silvia Rossi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

Dr Elisa Tamburini, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

For standard cloning based upon customer-provided material:

Internal price - 1.500 Euro/construct

External price - 2.500 Euro/construct

For in-house cloning and vector construction having COMT products as a starting material:

Please request quotation

Involves the process of transfection and selection of transient or stably expressing cell lines (cellular clones) using viral vectors provided by the customer, available at COMT, or produced by COMT upon customers’ request.

References

Dr Elisa Tamburini, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

Dr Serena Galati, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608

Dr Nicoletta Bertani, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-906003

Internal price - 1.500 Euro/line (customer-provided vectors)

External price - 2.000 Euro/line (customer-provided vectors)

Internal price – 2.000 Euro/line (vectors available at COMT)

External price - 2.500 Euro/line (vectors available at COMT)

Service 1

Qualitative PCR amplifications of DNA or RNA using primers designed and synthetized on demand or provided by the customer and electrophoretic analysis of the amplified products.

References

Dr Silvia Rossi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

Dr Federica Rizzi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903816

Internal price (up to 10 samples) - 250 Euro/gene/sample

Internal price (more than 10 samples) - 150 Euro/gene/sample

External price (up to 10 samples) - 400 Euro/gene/sample

External price (more than 10 samples) - 200 Euro/gene/sample

Service 2

Relative and absolute quantitative Real-Time PCR with primers and reference probes designed by COMT, or supplied by the customer (their specificity will be asserted by COMT).

Variant I – Single gene analysis based on 48- or 96- well platforms.

References

Dr Silvia Rossi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

Dr Serena Galati, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608

Dr Federica Rizzi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903816 (Relative quantitative Real-Time PCR)

Internal price (≤10 samples/gene) - 450 Euro/gene/sample

Internal price (>10 samples/gene) - 250 Euro/gene/sample

External price (≤10 samples/gene) - 600 Euro/gene/sample

External price (>10 samples/gene) - 300 Euro/gene/sample

(Absolute quantitative Real-Time PCR*)

Internal price - 750 Euro/gene/sample (customer-provided reference probe)

External price - 1.250 Euro/gene/sample (customer-provided reference probe)

Internal price - 1.200 Euro/gene/sample (COMT-provided reference probe)

External price – 1.750 Euro/gene/sample (COMT-provided reference probe)

*For more than 10 samples a 50% discount is applied.

Variant II – Multiple, high-throughput gene analysis - up to 364 genes evaluated simultaneously (microfluidity cards) - typically based upon platforms for the following “sample-gene” combinations (i.e. sample number x gene number): 1 sample x 364 genes; 4 samples x 48 genes, 8 samples x 24 genes. Statistics is based upon 2 internal replicates run in duplicate or triplicate.

References

Dr Silvia Rossi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

Dr Serena Galati, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608

Please request quotation

Conventional DNA microarray-based gene expression profiling on costumer-supplied cellular or tissue samples is offered relying upon the Agilent platform. One-color (a sample per array) and two-color (two samples per array) experiments are optionally offered. Statistics is based upon 2 spots/gene run in duplicate or triplicate.

The standard protocol entails:

1. extraction and purification of total RNA;
2. qualitative and quantitative analysis of RNA extracted by microfluidic systems;
3. fluorescent labelling of RNA with specific fluorophores and quantification of labelled RNA;
4. hybridization and slides image acquisition;
5. data processing.

References

Dr Nicoletta Bertani, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-906003

Internal price - 600 Euro/samples

External price - 1.000 Euro/samples

Please request quotation for tailored services

Protein knockdown by RNA interference technology, using naked or vector-contained siRNA or shRNA probes selected by COMT or based upon sequences provided by the customer. The service includes the design and validation of at least one functional probe and of specific control-probes directed to other segments of the RNA target. Upon request, it is also possible to receive “phenotype rescuing analyses” by BACs transfection or transfection of a specific recombinant plasmid.

References

Prof. Roberto Perris, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-906601

Dr Serena Galati, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608

Please request quotation